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The freely floating tissue sections containing corpus callosum lining around lateral ventricle and caudate nucleus were selected and treated with 1% Triton X-100 in PBS at 4 C overnight and incubated with primary antibody at a dilution of 1:200 in PBS containing 0.1% Triton X-100 and 1% HS at 4 C overnight

Spermatozoa possess a one-base excision repair (BER) enzyme upstream during their development, which is helpful for DNA repair

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