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The peak fractions were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (Cytiva) pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 1 mM TCEP)

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As a result, myocardial fibrosis, inflammation, and apoptosis were markedly attenuated, accompanied by improvements in mitochondrial function and calcium homeostasis ( At the in vitro cellular level, numerous studies have employed high-glucose or high-lipid-induced cardiomyocyte models to examine how 4-HNE dysregulation affects cardiomyocyte function, further substantiating its pathogenic role in DCM