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Ookhtens M, Maddatu T: Mechanism of changes in hepatic sinusoidal and biliary glutathione efflux with age in rats

The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

[DOI] [PMC free article] [PubMed] [Google Scholar] 11.Juan C.A., Prez de la Lastra J.M., Plou F.J., Prez-Lebea E