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After transfer, the membranes were blocked overnight at room temperature in PBS containing 3% BSA and 0.5% Tween 20 before being incubated for 1 h at room temperature with the primary Abs

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Physical examination revealed dry skin with atrophic areas, hyperpigmentation and poikiloderma on the face, neck, and chest, and pseudosyndactyly of the hands and feet (Fig

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