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These antibodies were used for immunofluorescence staining: NeuN(1:500, Abcam, Cat# ab177487), IGF1(1:500, Abcam, Cat# ab9572), IBA1 (1:500, Abcam, Cat# ab5076), c-Fos(1:500, CST, Cat#2250), CGRP(1:1000, SCBT, Cat#sc-57053), p-IGF1r(1:100, Abclonal, Cat#AP0367)

36 Moreover, CCS expression is regulated by cellular Cu status

It acts as an enzyme involved in nicotinamide adenine dinucleotide (NAD) consumption and intracellular signal transduction, while also functioning as an adhesion receptor (117)

While immunothrombosis can serve as a natural defense mechanism by immune cells like neutrophils and monocytes, dysregulated immune responses can create a thrombotic cascade