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After 01-Anisotropy Macro script has finished, navigate to 03-ROI Selection and press Run Using either the oval tool or the any shape tool draw regions of interest over the largest portion of where the sensor is appearing in the Para channel Each independent trial consists of at least five to more than 30 cells (530 technical replicates)

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) According to the protocol, total RNA was extracted from the large intestine with TRIzol (Life Technologies Japan Ltd., Tokyo, Japan) using a Dounce Tissue Grinder (DWK Life Sciences, LLC, NJ, USA), and 500 ng of RNA was reverse transcribed to complementary DNA using the Super Script III First-Strand Synthesis Super MIX kit (Life Technologies Japan Ltd.)

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