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Cultures were then incubated with Alexa Fluor 555 donkey anti-mouse IgG (#A31570

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Paq5000 DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) was used for amplification from swab samples, and 5% dimethyl sulfoxide (DMSO) was added to the reaction to improve the concentration of the amplified products from the metagenomic template

Food and Drug Administration classifies castoreum as generally regarded as safe, and a 2007 safety assessment published in the International Journal of Toxicology concluded that a long historical use of castoreum extract as a flavoring and fragrance ingredient has resulted in no reports of human adverse reactions. I would try it, Francl says