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Mitochondrial Fluorescent Probe Staining Analysis Mitochondrial staining was performed with the mitochondrial probe MitoTracker Red CM-H2XRos (Invitrogen, USA) according to protocols provided by the manufacturer

In our study, the incidence of LBP upon lumbar extension was three times higher than that of LBP upon lumbar flexion on check-up evaluations, indicating that increased force on the posterior lumbar spine elements contributes to the occurrence of LBP [27, 28]

That means raises NAD-related markers is not the same as proven to reverse aging or guaranteed to fix fatigue. For medical decision-making, that distinction is essential