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Another study also elucidated that AARS1 can function as a lactate sensor, catalyzing the generation of lactate-AMP, and mediating the lactylation of p53, and the lactylation of p53 will inhibit its liquid-liquid phase separation, DNA binding capabilities, and ultimately lead to a decrease in transcriptional activity, fostering the onset and development of tumors

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At the methodological level, isotope tracing technologies can directly resolve actual metabolic flux changes, single-cell and spatial multi-omics can help identify cell-cell interactions and substrate competition in the tissue microenvironment, and genome-scale metabolic modeling can be used for priority ranking and assessment of targetability