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HBECs or BEAS-2B cells grown on 6-well culture plates were lysed in RIPA buffer (Thermo Fisher Scientific, 89900) containing a protease inhibitor cocktail (Roshce, 11697498001) and 1 mM sodium orthovanadate (Wako, 13721-39-6)

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On the other hand, there are multiple factors behind nicotine dependence or depression, such as some personality predispositions, which in turn can be an outcome of mechanisms associated with neurobiological conditioning of cognitive disorders progress