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Following incubation for 60 min, the reaction was terminated by adding 1% formic acid solution and the ATP and -glutamylcysteine levels were measured using RapidFire300 mass spectrometry (Agilent Technologies, Inc., Santa Clara, CA, USA) coupled with API4000 triple quadrupole mass spectrometer (AB Sciex, Framingham, MA)

Abstract Legumes of the genus Medicago are agronomically important forage crops that also enhance soil fertility through biological nitrogen fixation