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The debenzylated product was precipitated in hexane, dried using a centrifuge and vacuum, dissolved in basic water, and purified by dialysis with a dialysis membrane (molecular weight cut-off 3.5 kDa) against deionized water (DIW) for three days before the lyophilization for removing water and collecting the final product

Thymosin 1 modifies, enhances, and restores immune function, especially by increasing T-cell numbers and activity

Perovic et al., 2019)

While initially proposed to have HAT activity 23 , previous work 24,25 , along with our data shown here, show that eukaryotic HAT-like domains cannot bind acetyl-CoA, classifying them as pHATs of unknown function