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Small interfering RNA For siRNA knockdown experiments, cells were transfected (DharmaFECT 1, Horizon Discovery) with ON_TARGETplus SMART pools (Horizon Discovery) targeting individual ACSL family members, GPX4 (Catalog ID: L-011676-00), DGAT1 (Catalog ID: L-003922-00), DGAT2 (Catalog ID: L-009333-00), ATGL (Catalog ID: L-009003-01) or a non-targeting siRNA pool (Pool #1, Catalog ID: D-001206-13, Horizon Discovery)

01 , p 90%) were reported in the GSH group, ensuring reliability of the findings

This effect seems to be independent of mu opioid peptide receptors (MOP), as it was not reversed by the MOP antagonist naltrexone, nor mimicked by the MOP agonist methadone [65]

This is a component of the electron transport chain and participates in aerobic cellular respiration, generating energy as ATP