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Levels of HMGB1::Lucia in the supernatant can be readily monitored by measuring the light signal produced after the addition of QUANTI-Luc 4 Lucia/Gaussia, a Lucia luciferase detection reagent ( see figure )

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While the active group did have significantly improved self-report of subjective cravings, withdrawal, and overall distress, the COWS itself was not significantly different between groups at the 2-hour endpoint