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Both methods are equally safe

The findings revealed a concentration-dependent biphasic effect: water permeability decreased at low concentrations of curcumin (up to 2 mol%), while at higher concentrations (above 3 mol%), permeability increased

For lysates preparation ( 31 ), cultures were centrifuged (10,000 g, 15 minutes), washed twice with potassium phosphate buffer (100 mM, pH 7.0), resuspended in borate buffer (50 mM, pH=8.4), and broken by mechanical lysis with glass beads in a tissue lyser (Qiagen, Courtaboeuf, France) 10 minutes, 50 Hz

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