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Seventy micrograms of AAV shuttle vector, 200 g Delta F6 helper plasmid and 70 g AAV2/8 Rep/Cap plasmid were prepared with EndoFree Plasmid Maxi kit (QIAGEN) and transfected into 15-cm plates of HEK293T cells using PEI transfection reagent (Sigma-Aldrich)

Wormwood blocks the proinflammatory cytokine TNF-, which may account for its effectiveness at maintaining remission in Crohns patients who tapered off their medications

Acknowledgements Editorial Staff Elizabeth Jean Betsch, ELS Amy Zierler, BA Medical Information Services Corinne Holubowich, BEd, MLIS Kellee Kaulback, BA(H), MISt Clinical Expert Advisory Panel: Appropriate Utilization of Vitamin B12 Testing for Neurocognitive-Based Indications