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Acknowledgements This work was supported by the National Science Foundation of China (21405071, 21535002, 21227008), Special Funds for the Construction of Taishan Scholars (grand No

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Since bacteria exhibit different modes of response to different antibiotic concentrations,[] we used moxifloxacin at MBC levels to maintain the antibiotic concentration always high, with negligible changes in its concentration during the exposure, reported earlier.[] We did not study antibiotic response at single-cell level as antibiotic-exposed single cells in isolation respond differently to antibiotics, unlike the single cells in a population, depending on the antibiotic concentration and exposure duration.[] Formation of three reactive oxygen species in the moxifloxacin-exposed Mycobacterium smegmatis cultures The genetic resisters to moxifloxacin were formed from the cells in the ASP containing the three ROS and Fe (II) at significantly elevated levels