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Other protein sources, such as nuts, seeds, grains and beans, contain some but not all essential amino acids and are therefore referred to as incomplete

In contrast, the gdh3 / gdh3 mutant showed overall higher concentrations of intracellular metabolites than the wild-type strain when grown in MM medium, except for 2-aminobutyrate and norvaline.When grown in a proline medium, the gdh3 / gdh3 mutant revealed a significantly higher concentration of glutamate family metabolites (e.g., glutamate, glutamine, and proline) than the wild-type, whilst asparagine, histidine, phenylalanine, valine, leucine, tyrosine, and aspartate family metabolites (e.g., aspartate, isoleucine, and threonine), as well as all saturated fatty acids detected, were at lower concentrations in the gdh3 / gdh3 mutant

Consistent with previous studies in mice, we also observed an increase in branched-chain amino acids (Leucine, Isoleucine, and Valine) in the brains of both DL and CR-fed female mice 12,83