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The thermal cycling was run using the BioRad CFX96 Real Time Detection System programmed as follows: one cycle of heating at 95C for 3 min, 36 cycles of denaturation at 93C for 30 s, annealing at 62C for 45 s, and elongation at 72C for 40 s

However, problems arise when this type of stress is ongoing and the body is constantly suppressing thyroid function to preserve its resources

For example, T cell activation induces the expression of ASCT2 (encoded by Slc1a5 ), which mediates glutamine uptake and further leads to the activation of mTORC1 [211]