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Under these conditions, although adduct formation is quite possible, a substantial amount of substrate remains available for the ChaC1 enzyme to catalyze the reaction

Briefly, after isolation from the adult mouse, CMs were placed in a chamber under an inverted microscope (Olympus, IX-70) in contractile buffer (containing 135 mM NaCl, 10 mM HEPES, 1.0 mM MgCl 2 , 1.0 mM CaCl 2 , 10 mM glucose and 4.0 mM KCl (pH = 7.4))