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SKU: 52436708054
Price: 28.24 USD
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Description

To assess the presence of potential off-target effects of the CRISPR/Cas9 sgRNA sequences used to generate the Mettl9 KO cell lines, we predicted all putative off-target sites using Cas-OFFinder 154 , allowing up to five mismatches across the 20-base target sequence (which is the highest value permitted by the program)

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

Quantification of 2HG enantiomers 2HG quantification in SCD erythroblasts was performed as we previously described 24 by liquid chromatographymass spectrometry (LC-MS/MS) after derivatization using N-(p-Toluenesulfonyl)-L-phenylalanyl Chloride (TSPC) on the LC-ESI-MS/MS system