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Given that the degradation of KEAP1 encompasses two distinct pathways, namely autophagy 29 and proteasomal degradation 30 , we employed the protein synthesis inhibitor cycloheximide (CHX), the proteasome inhibitor MG132, and the autophagy inhibitor chloroquine (Chlq) to further explore the underlying mechanism in macrophages (Fig

Bead-bound GST proteins were pelleted, washed three times in bacterial lysis buffer, and stored at 80 C

gRNA and Cas9 are injected into embryos, followed by implantation into foster mothers to generate founders

Prevention of neural tube defects: results of the Medical Research Council Vitamin Study