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(3) The combined structural data and all-atom molecular dynamics simulations indicate that Arabidopsis SNX1 has a reduced membrane affinity compared to mouse SNX1, primarily due to differences in its AH domain, which in turn restricting the formation of longer tubular structures relative to those formed by mouse SNX1

use of degenerate N,N,G/T triplets allows for systematic and easy generation of a full range of possible natural amino acids (for a total of 20 amino acids) into each and every amino acid position in a polypeptide (in alternative aspects, the methods also include generation of less than all possible substitutions per amino acid residue, or codon, position)

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