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Oxygen Consumption Assay Anaerobic buffers for this experiment were degassed on a Schlenk line by purging 50 mL of buffer with ultrapure argon for 2 h then immediately transferred to a glove box for storage

These findings suggest that Piezo1 is a potential target for treating neuroinflammation

Reagents Internal standards in the form of d4-labelled stable isotopes of estrone (E1), estradiol (E2), and estriol (E3), and d9-labelled isotope of progesterone were purchased from Steraloids (Newport, RI)